The adult blood system is maintained by cells residing the bone marrow known as haematopoietic stem and progenitor cells (HSPCs). These cells can differentiate and make different mature blood cell types.To better understand the changes in gene expression as these cells differentiate towards the mature blood lineages, we profiled 44,802 individual HSPCs using a droplet-based sequencing method. Cells were collected from mouse bone marrow in two gates: Lin- c-Kit+ (LK) and Lin- Sca-1+ c-Kit+ (LSK). Gene expression patterns in the resulting transcriptional landscape can be visualised in the force-directed graph layout of the single-cell profiles below.
This dataset has been published in Dahlin JS*, Hamey FK*, et. al., (2018), Blood.
You can find a Python notebook with the analysis and the corresponding data in the links below:
Check out the Python notebook.To investigate the effect of a signalling mutation on the haematopoietic transcriptional landscape we also profiled LK cells from W41/W41 mice, which have impared c-kit kinase activity. Clustering was performed on WT LK cells, and cells from the mutant mice projected onto the WT clusters. Gene expression can be visualised below in both the force-directed graph layouts and in violin plots of the expression in each cluster.